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Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy
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Researchers used dark-field hyperspectral microscopy to track the distribution of 2-micrometer polystyrene microspheres in mosquito larvae without relying on fluorescence, finding that histological processing quenches fluorescence and alters spectral properties, and demonstrating that enhanced dark-field microscopy coupled with hyperspectral imaging is a reliable fluorescence-free method for mapping microplastic biodistribution in complex tissues.
Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.
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Researchers developed a method to detect unlabeled nanoplastics within Daphnia magna using enhanced darkfield hyperspectral microscopy combined with a post-exposure histological labeling process. The study presents a new approach for identifying nanoplastic particles in model organisms without requiring pre-labeled or fluorescent plastics.
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Researchers developed a label-free imaging technique to visualize and count nanoplastic particles that accumulate inside human cells, using enhanced dark-field microscopy combined with hyperspectral imaging. The method successfully tracked polystyrene nanoplastics entering cells over time and measured accumulation rates without needing fluorescent labels. This tool could improve the accuracy of future studies assessing how nanoplastics build up in human tissue and what concentration levels may pose health risks.
Label-free detection of micro- and nanoplastics using dark-field hyperspectral and atomic force microscopies
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Researchers demonstrated that dark-field hyperspectral microscopy could visualize and spectrally identify polystyrene, polymethacrylate, and melamine formaldehyde particles (100 nm to 2 µm) in living Caenorhabditis elegans in vivo, while atomic force microscopy in PeakForce Tapping mode resolved internalized versus surface-bound polystyrene particles (down to 500 nm) in human skin fibroblasts. Deep learning algorithms applied to enhanced dark-field images further enabled automated classification of pigmented microplastics, demonstrating a label-free toolkit for detecting and differentiating particles in biological systems.
Dark-field hyperspectral microscopy for label-free microplastics and nanoplastics detection and identification in vivo: A Caenorhabditis elegans study
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Researchers demonstrated that dark-field hyperspectral microscopy can visualize and chemically identify nano- and microplastics (down to 100 nm) in live C. elegans nematodes without labeling, differentiating multiple polymer types simultaneously within intestinal tissue.
Dark-field/hyperspectral microscopy for detecting nanoscale particles in environmental nanotoxicology research
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Researchers reviewed enhanced dark-field microscopy combined with hyperspectral imaging as a non-destructive optical technique for detecting and chemically characterizing nanoscale particles — including nanoplastics — in live, wet biological samples where electron microscopy is impractical.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.