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Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy
Summary
Researchers used dark-field hyperspectral microscopy to track the distribution of 2-micrometer polystyrene microspheres in mosquito larvae without relying on fluorescence, finding that histological processing quenches fluorescence and alters spectral properties, and demonstrating that enhanced dark-field microscopy coupled with hyperspectral imaging is a reliable fluorescence-free method for mapping microplastic biodistribution in complex tissues.
Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.