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Fluorescence-Free Tracking of Polystyrene Microplastics in Mosquito Larvae Using Dark-Field Hyperspectral Microscopy
Colloid Journal2024
1 citation
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Score: 35
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Yulia A. Frank,
Ilnur Ishmukhametov,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Ilnur Ishmukhametov,
Yulia A. Frank,
Yulia A. Frank,
Marina Kryuchkova,
Rawil Fakhrullin
Yulia A. Frank,
Anna A. Varenitsina,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Ilnur Ishmukhametov,
Yulia A. Frank,
Yulia A. Frank,
Ilnur Ishmukhametov,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Anna A. Varenitsina,
Anna A. Varenitsina,
A. V. Simakova,
A. V. Simakova,
Rawil Fakhrullin
Ilnur Ishmukhametov,
Ilnur Ishmukhametov,
A. V. Simakova,
A. V. Simakova,
Yulia A. Frank,
Rawil Fakhrullin
Rawil Fakhrullin
Yulia A. Frank,
Yulia A. Frank,
Rawil Fakhrullin
Rawil Fakhrullin
Rawil Fakhrullin
Rawil Fakhrullin
A. V. Simakova,
Anna A. Varenitsina,
A. V. Simakova,
Yulia A. Frank,
Yulia A. Frank,
Vadim V. Yartsev,
Rawil Fakhrullin
Yulia A. Frank,
Yulia A. Frank,
Yulia A. Frank,
Vadim V. Yartsev,
D. A. Nadueva,
D. A. Nadueva,
Rawil Fakhrullin
Yulia A. Frank,
Anna A. Varenitsina,
Yulia A. Frank,
Rawil Fakhrullin
Yu.V. Andreeva,
Rawil Fakhrullin
Summary
Researchers used dark-field hyperspectral microscopy to track the distribution of 2-micrometer polystyrene microspheres in mosquito larvae without relying on fluorescence, finding that histological processing quenches fluorescence and alters spectral properties, and demonstrating that enhanced dark-field microscopy coupled with hyperspectral imaging is a reliable fluorescence-free method for mapping microplastic biodistribution in complex tissues.
Using dark-field hyperspectral microscopy, we analyzed the distribution of fluorescently-labelled polystyrene microspheres with a size of 2 μm in mosquito larvae and observed the quenching of fluorescence and changes in spectral properties due to histological processing. We propose enhanced dark-field microscopy coupled with hyperspectral imaging as a versatile method for analysing polymer colloids (microplastics) biodistribution in complex tissues.