Nanoplastic Sample Cleanup by Micro-Electromembrane Extraction across Free Liquid Membranes

Sample preparation techniques enabling the separation and cleanup of nanoplastics removing other components present in complex sample matrices are scarce. Herein, micro-electromembrane extraction (μ-EME) has been explored for this purpose based on the extraction of nanoplastic particles across a free liquid membrane (FLM). The extraction unit is based on a perfluoroalkoxy tube sequentially filled with the acceptor solution (20 μL 5 mM phosphate buffer, pH 10.7), FLM (10 μL 1-pentanol), and donor solution (20 μL sample/standard solution). Sulfonated polystyrene beads (200 nm particle size) were selected as a model mimicking negatively charged nanoplastics. At 700 V, nanoplastics transferred from the donor solution into the FLM before moving across the FLM into the acceptor solution. Quantitative nanoplastic measurements after μ-EME were performed by injecting the acceptor solution into a capillary electrophoresis system with diode array detection. μ-EME allowed the rapid nanoplastic sample cleanup, requiring an extraction time of just 90 s and obtaining a nanoplastic transfer yield through the FLM of 60% with RSD values below 9%. The μ-EME technique enabled the efficient sample matrix cleanup of nanoplastics spiked in different tea matrices. Nanoplastic transfer yield through the FLM for black tea and flavored tea matrices were 56% and 47%, respectively, with complete sample matrix removal of UV-absorbing compounds.