A study on the effect of fluorescently stained micro(nano)plastics on the full life history of Skeletonema costatum

As a new type of environmental pollutant, micro(nano)plastics have become a research hotspot in recent years, and their effects on the full life history of marine microalgae have not been studied. To investigate the effects of micro(nano)plastics on the growth, photosynthesis, physiological morphology and interaction of microalgae during the full life cycle, we selected fluorescently stained polystyrene (PS) plastic microbeads as the target pollutant. By sampling and testing the growth rate, photosynthesis and physiological morphology parameters of algal species, the influence of different concentrations of PS (10, 50 and 100 mg/L) and different particle sizes (0.1, 0.5 and 1 μm) on the full life history of Skeletonema costatum (S. costatum) was investigated. The results showed that after adding PS (particle sizes of 0.5 and 1 μm), the response of S. costatum showed a dual character, while adding the same kind of microplastics (MPs) with a particle size of 0.1 μm inhibited S. costatum throughout the full life cycle. Compared with previous studies, short-term experimental data may overestimate the true ecological risks of MPs. In addition, 0.1 μm fluorescent-stained MPs obviously accumulated around the microalgae, indicating that MPs mainly adhered to the surface of algal cells and may enter the food chain by direct or indirect ways, which can cause negative effects on the aquatic ecosystem. This study supports a more accurate assessment of the true risk of MPs to marine aquatic ecosystems.