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Toxicological assessment of benzo(a)pyrene - coated PET nanoplastics in vitro on a 3D model of the human bronchial epithelium

Zenodo (CERN European Organization for Nuclear Research) 2024 Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Safaa Mawas, Safaa Mawas, Safaa Mawas, Safaa Mawas, Raquel Portela, Raquel Portela, Dorian Miremont, Raquel Portela, Dorian Miremont, Safaa Mawas, Dorian Miremont, Dorian Miremont, Safaa Mawas, Raquel Portela, Víctor Alcolea-Rodriguez, Linh Chi Bui, Linh Chi Bui, Linh Chi Bui, Linh Chi Bui, Raquel Portela, Víctor Alcolea-Rodriguez, Stéphanie Devineau, Justine Renault, Sonja Boland Sonja Boland Justine Renault, Justine Renault, Justine Renault, Charlotte Izabelle, Stéphanie Devineau, Charlotte Izabelle, Charlotte Izabelle, Charlotte Izabelle, Raquel Portela, Raquel Portela, Víctor Alcolea-Rodriguez, Raquel Portela, Raquel Portela, Víctor Alcolea-Rodriguez, Stéphanie Devineau, Stéphanie Devineau, Raquel Portela, Stéphanie Devineau, Dorian Miremont, Víctor Alcolea-Rodriguez, Dorian Miremont, Víctor Alcolea-Rodriguez, Víctor Alcolea-Rodriguez, Stéphanie Devineau, Raquel Portela, Stéphanie Devineau, Raquel Portela, Stéphanie Devineau, Stéphanie Devineau, Raquel Portela, Raquel Portela, Raquel Portela, Julien Dairou, Julien Dairou, Julien Dairou, Julien Dairou, Stéphanie Devineau, Stéphanie Devineau, Raquel Portela, Raquel Portela, Sonja Boland Sonja Boland Raquel Portela, Stéphanie Devineau, Charlotte Izabelle, Raquel Portela, Sonja Boland

Summary

Researchers assessed the in vitro toxicity of PET nanoplastics coated with benzo(a)pyrene on airway epithelial cells, simulating inhalation of pollutant-loaded particles. The combination of nanoplastics and PAH contamination produced stronger toxic effects than either stressor tested alone.

Polymers

PE PET

Body Systems

Immune Respiratory

Study Type In vitro

Exposure to airborne micro/nanoplastics (MNPs) may pose a potential threat to human health, as several studies have detected MNPs in the respiratory tract [1,2]. These MNPs may act as carriers of airborne pollutants, like polycyclic aromatic hydrocarbons (PAHs). The aim of our study is to perform a toxicological assessment of co-exposure to airborne MNPs and a PAH known for its toxicity, benzo(a)pyrene (BaP). To evaluate this, we developed the coating of polyethylene terephthalate NPs (PET, 70 nm) with BaP and assessed their toxicity in vitro on a 3D model of the human bronchial epithelium [3]. The Calu-3 cell line was exposed to PET-NPs or BaP alone or in combination at the air liquid interface. Transmission electron microscopy has shown that PET-NPs can be internalized by the bronchial epithelium. No effects on the barrier integrity (Transepithelial electrical resistance) and cell viability (AlamarBlue assay) were observed after 6h, 18h and 24h of exposure. BaP and BaP-coated PET-NPs induced the overexpression of cytochromes P450 mRNAs. This suggests that BaP is bioavailable when adsorbed on PET-NPs. We are currently assessing the metabolization pathway of BaP desorbed from PET-NPs in the bronchial epithelium. Intrestingly, BaP-coated PET-NPs but not BaP alone induced the overexpression of mucins, anti-oxidants and inflammatory cytokines mRNAs after 6h, 18h and 24h of exposure. Repeated exposures to BaP-coated PET-NPs are ongoing to assess the long-term effects over 28 days of daily exposure (5 days/week) to PAH-contaminated PET-NPs. Our results emphasize the importance of studying the interaction of MNPs with airborne contaminants and its influence on their toxicity. This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 965367 (PlasticsFatE). 1. Jenner et al. Sci. Total Environ. 831, 154907 (2022). 2.Uoginte et al. Heliyon 9, (2023). 3.Sanchez-Guzman et al. Sci. Rep. 11, 6621 (2021). Also see: https://micro2024.sciencesconf.org/558610/document

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