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Razvoj mjernog postupka za detekciju mikro i nanoplastičnih čestica u uzorcima tkiva
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This thesis developed and evaluated a measurement procedure for detecting micro- and nanoplastic particles in tissue samples, reviewing tissue digestion methods and applying optical microscopy and spectroscopy for particle identification. Micro- and nanoplastic particles were successfully identified in tissue samples alongside other contaminants, advancing methods for studying internal human and animal microplastic accumulation.
With the mass accumulation of plastic pollution in the environment, problems are present even on the micro and nano scale. Micro and nanoplastic (MNP) particles with a diameter of less than one millimeter, mostly generated by the degradation and wearing down of larger plastic parts, are accumulating in the ecosystem and reaching the human body in different ways. The potential of the particles to adsorb harmful substances poses a big threat to ecosystems and human health. Analytical chemistry offers essential tools for the detection, quantification, and characterization of small particles, enabling researchers to study their distribution, composition, and impact. The most used methods are different types of microscopy and spectroscopy. This work provides the process of preparing and analyzing different tissue samples for MNP research. A review of the most common tissue digestion methods was made, along with two analytical methods that were used to detect, quantify, and characterize MNPs: optical microscopy and spectroscopy. MNP particles were found and identified in the tissue samples, along with present contaminants.
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