Plastisphere in a low-pollution mountain river: Influence of microplastics on survival of pathogenic bacteria

Microplastics (MPs) are found even in remote and low-pollution freshwater ecosystems. However, the microbial communities associated with MPs in these environments remain poorly understood. We characterized the plastisphere in a low-pollution riverine ecosystem and evaluated the influence of different MPs in the persistence of pathogens in such environments. A mixture of MPs (MPs Mix) composed of polyethylene (PE), polypropylene (PP) and polyethylene terephthalate (PET), was submerged at three locations (L1, L2 and L3) in the river. For comparison purposes, water and sand communities were also analyzed. Our results revealed distinct bacterial communities on MPs compared to those in water or on the natural substrate (sand). However, the resemblance between the plastisphere and communities on natural particles was higher than what has been reported for polluted ecosystems. Although pathogens were predominantly enriched in the water, a few genera (e.g. Acinetobacter, Legionella and Mycobacterium) were enriched in the plastisphere. The abundance of antibiotic resistance genes did not differ significantly between water, sand, and MPs. The influence of different MPs (PE, PP, PET) on the persistence of antibiotic-resistant pathogens (i.e. cefotaxime-resistant Escherichia coli and meropenem-resistant Enterobacter kobei) in unpolluted water was assessed in microcosms. Significant differences were observed between the microcosms with MPs and those with natural particles (sand), after a 36-day exposure. A significantly higher persistence of the pathogens was registered in microcosms with PE and PET. Our results provide new insights into the plastisphere in non-pollution environments and demonstrate that even in these settings, MPs can facilitate the survival and dissemination of pathogens.