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Adverse effects of environmentally relevant concentration of microplastics on gill epithelium permeability in the euryhaline Mediterranean killifish Aphanius fasciatus
Summary
Researchers exposed Mediterranean killifish to environmentally relevant concentrations of polystyrene microplastics for 15 days and examined the effects on gill function. They found that microplastics accumulated in the gills and disrupted the permeability of gill epithelium, potentially impairing the fish's ability to regulate salt and water balance. The findings suggest that microplastic exposure could compromise the ability of estuarine fish to tolerate the naturally fluctuating salinity of their habitats.
Estuaries and lagoons are characterized by fluctuating salinity and significant amounts of microplastics (MPs) and are increasingly subjected to various anthropogenic pressures. We investigated whether the accumulation of MPs in the gills of fish inhabiting these fragile ecosystems alters osmoregulation and, consequently, their ability to tolerate fluctuating salinity. The effects of a 15-day exposure to an environmentally relevant concentration (20 μg/L) of spherical polystyrene microplastics (PS-MPs) with a diameter of 5 μm were assessed in the Mediterranean killifish Aphanius fasciatus, focusing on tissue and gene expression changes related to factors of paracellular and transcellular permeability of the gill epithelium during the transition from seawater to freshwater. Our results revealed that PS-MPs indirectly impaired osmoregulation, particularly in fresh water, through their toxic effects on the gill tissue. Toxicity was evidenced by epithelial lifting, a decrease in the proportion of secondary lamellae available for gas exchange, and upregulation of superoxide dismutase and heat shock protein genes. Furthermore, exposure to PS-MPs directly affected gill epithelial permeability by maintaining relatively high paracellular permeability through the downregulation of claudin 3 and by modifying the expression of the transcellular transporter Na+/K+-ATPase and cystic fibrosis transmembrane conductance regulator in the gill epithelium. Overall, these findings confirm the toxic effects of PS-MPs on gill tissue and demonstrate, for the first time, that environmentally relevant concentrations of MPs adversely affect gill epithelium permeability during decreased salinity acclimation in the euryhaline fish A. fasciatus.