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Identification and Characterization of New Hafnia Strains from Common Carp (Cyprinus carpio), Potentially Possessing Probiotic Properties and Plastic Biodegradation Capabilities

International Journal of Molecular Sciences 2025 4 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 48 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Luka Dragačević, Darya Tsibulskaya, Milan Kojić, Nevenka Rajić, Aleksandar Nikšić, Mina Popović

Summary

Researchers isolated and characterized two novel Hafnia bacterial strains from the gut of common carp (Cyprinus carpio), identifying genes associated with both probiotic properties and microplastic degradation capabilities. Next-generation sequencing confirmed the strains carry ClpB heat shock protein and MP-degrading genes, presenting them as candidate organisms for simultaneous probiotic and bioremediation applications.

Finding and characterizing new bacterial strains, including probiotic strains, is a crucial task in today's world to expand the genetic data pool and identify new genes. In this study, we investigated the gut microbiota of one industrial species, Cyprinus carpio, and identified representatives of various microbial genera, including Citrobacter, Serratia, Bacillus, Enterococcus, and Kocuria. Notably, we discovered two strains of Hafnia with potentially probiotic properties. We conducted next-generation sequencing (NGS) of these strains, described their antibiotic resistance and antibacterial activity, and compared them with other representatives of the Hafnia genus. These strains, characterized by rapid growth, the presence of the ClpB heat shock protein gene, and genes associated with microplastic degradation, provide a promising basis for further research, including studies on their potential application in plastic biodegradation.

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