Synergistic neurotoxicity of clothianidin and photoaged microplastics in zebrafish: Implications for neuroendocrine disruption

Microplastics (MPs), widely found in aquatic environments, pose a growing threat to environmental and biological health due to their complex interactions with pollutants and microorganisms. This study investigates the adsorption characteristics of clothianidin (CLO) on polystyrene (PS) and photoaged polystyrene (P-PS) and explores the neurotoxic effects of CLO combined with PS/P-PS in larval zebrafish (Danio rerio). Adsorption kinetics and isotherms showed that P-PS exhibited a higher adsorption capacity and faster equilibrium compared to PS, indicating the significant role of photoaging in enhancing CLO adsorption. Exposed to CLO combined with PS/P-PS resulted in reduced locomotor activity, particularly in the P-PS + CLO group, suggesting amplified neurotoxicity due to P-PS. Analysis of the hypothalamic-pituitary-interrenal (HPI) axis revealed elevated levels of adrenocorticotropic hormone (ACTH) and cortisol, along with downregulated expression of stress-related genes in co-exposed zebrafish, indicating disruption of neuroendocrine function. Neurotransmitter analysis showed significant changes in acetylcholine (ACh), dopamine (DA), serotonin (5-HT), and γ-aminobutyric acid (GABA) levels, further confirming the neurotoxic impact of co-exposure. The findings highlight the synergistic neurotoxicity of CLO and photoaged MPs, with potential implications for aquatic ecosystems. This study advances the field of environmental science by addressing critical knowledge gaps in pollutant-microplastic interactions, providing a foundation for developing targeted mitigation strategies and enhancing ecological risk management frameworks.