Enhancing microplastic removal and nitrogen mitigation in constructed wetlands: An earthworm-centric perspective

The presence of microplastics (MPs) in wastewater poses significant threats to ecosystems. Although constructed wetlands (CWs) demonstrate effective removal of microplastics, their efficiency is often limited by various environmental variables and system equilibrium factors. This study introduces Eisenia fetida to enhance the ecological performance of constructed wetlands and improves their removal efficiency. The findings revealed that the addition of earthworms significantly increased the removal efficiencies of polylactic acid (PLA) microplastics, chemical oxygen demand, total nitrogen, and ammonium nitrogen, with respective improvements of 13.5 %, 8.4 %, 9.7 %, and 10.5 %, respectively. Notably, the ingestion of polylactic acid microplastics by earthworms led to a substantial increase in the abundance of microorganisms, such as Actinobacteria, that were associated with microplastic degradation. Furthermore, microbial communities involved in nitrogen cycling were notably enriched, with a 12.4 % increase in nitrogen-fixing microbes and a 4.3 % increase in nitrifying microbes. These findings suggested that earthworms, through the restructuring of their gut microbial communities, not only facilitated efficient polylactic acid degradation but also enhanced nitrogen cycling processes. This provides a novel ecological mechanism for improving pollutant management and nutrient recycling in constructed wetlands.