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Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications

MethodsX 2025
Mutsumi Iizuka, Atsuko Amano, Takuya Itaki

Summary

Researchers developed a sediment sampling protocol using a Kinoshita-type grab sampler with stainless steel containers and aluminum tubes to collect microplastics and macroplastics from 133 marine sediment samples at depths of 20-800 m during the GB24 geological survey cruise, minimizing contamination while maintaining sample integrity.

Study Type Environmental

This study describes a sediment sampling protocol using a Kinoshita-type grab (K-grab) sediment sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. During the GB24 geological survey cruise aboard the , 133 surface sediment samples were collected from depths of 20-800 m. The K-grab, equipped with a head-slide weight mechanism, enhanced sampling efficiency across various sediment types. For microplastics, stainless steel containers and J-shaped aluminum tubes minimized contamination while maintaining sample integrity. Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination. These results demonstrate that the K-grab is a reliable tool for microplastic and macroplastic analysis, providing valuable data on plastic pollution in marine sediments.•This study describes a sediment sampling protocol using a grab sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments.•During the survey, 133 surface sediment samples were collected from depths of 20-800 m, with microplastics handled using J-shaped aluminum tubes and stainless steel containers to minimize contamination while maintaining sample integrity.•Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination.

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