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Polystyrene microplastics (PS-MPs) harness copper presence and promote impairments in early zebrafish (Danio rerio) larvae: Developmental, biochemical, transcriptomic approaches and nontargeted metabolomics approaches

Environmental Research 2025 7 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Liqiao Zhang, Dongqiu Guo, Xinrui Dong, Longxiao Liu, Mengqi Jiang, Linlin Song, Xia Wang

Summary

This zebrafish study found that polystyrene microplastics combined with copper produced worse toxic effects than either pollutant alone, causing developmental problems, oxidative stress, immune disruption, and nerve damage in larvae. The combination also disrupted the fish's metabolism in ways that neither pollutant caused independently. This is concerning because microplastics readily absorb heavy metals like copper in the environment, meaning organisms are often exposed to both together.

Polymers
Body Systems

Due to their large specific surface area and strong hydrophobicity, microplastics (MPs) are highly susceptible to adsorb environmental pollutants, of which heavy metals (HMs) are the most representative inorganic pollutants. However, there is controversy in different studies as to whether the toxic effects of the combined action of MPs and HMs on zebrafish larvae are antagonistic or synergistic. Herein, we firstly evaluated the combined effects of 10 μm polystyrene MPs (PS-MPs) on zebrafish larvae after exposure for 96 h at two different concentrations (5 mg/L, 0.5 mg/L) and Cu2+ (0.05 mg/L). Our study primarily showed that the combined exposure of PS-MPs and Cu2+ could induce developmental toxicity, oxidative stress, immunotoxicity and neurotoxicity. Furthermore, LC/MS-based nontargeted metabolomics analysis demonstrated that the combined exposure of PS-MPs with Cu2+ induced metabolic disturbances. Furthermore, our results showed that the combined exposure of 10 μm PS-MPs with Cu2+ exhibited a synergistic effect on the toxicity of zebrafish larvae. In conclusion, this study provides a reference for future research related to combined exposure of PS-MPs and Cu2+ on fish.

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