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The FOXO pathway mediates a conserved mechanism of antioxidant defense against microplastic-induced toxicity in Aurelia coerulea polyps and mouse liver

Ecotoxicology and Environmental Safety 2025 2 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Xiaoxiao Chen, Yina Zhu, Feng Wu, Liang-zhi Li, Yi Wang, Blessing Danso, Zhixiao Yang, Xiao Peng, Xiao Peng, Marina Pozzolini, Qinglong Tang, Xiao Liang, Xiaojie Dai, Xiaojie Dai

Summary

Researchers exposed both jellyfish polyps and mice to polystyrene microplastics and found that both species suffered tissue damage, oxidative stress, and activated similar antioxidant defense pathways through what is known as the FOXO signaling pathway. The gene sequences involved in this response were nearly 50% similar between the two very different species, suggesting a conserved biological defense mechanism. The study provides evidence that the cellular response to microplastic damage may be shared across a wide range of animal life.

Body Systems
Models

Microplastics are emerging as a critical class of pollutants with a pervasive presence in the atmosphere, oceans, and terrestrial environments, raising global concern. However, it is unclear whether there are conserved mechanisms for the response of microplastic damage to different species. Aurelia coerulea polyps and mouse models were exposed to microplastic environments to assess their growth effects and mechanisms. The similarities and differences in the defence mechanisms of the two species after exposure to microplastics were analysed by means of phenotypic observations, transcriptome sequencing, pathological sections, oxidative stress indicators, explosions and molecular docking. Exposure to 200 mg/L of PS-MPs resulted in morphological changes, tissue damage, and oxidative stress in polyps. Exposure to 600 mg/L of PS-MPs led to increased toxicity in the mouse liver, including rupture and apoptosis of mitochondria and nuclei in hepatocytes, as well as oxidative stress. Meanwhile, we found that their antioxidant defense and DNA damage repair pathways were significantly altered. In addition, we found that the same loci existed in the foxo pathway in both species, and the enriched gene sequences were 48.33 % similar. Molecular docking showed conserved regions between polyps NLK and mouse Mapk13, indicating similar kinase activities, but overall structural and functional differences suggest species-specific. This finding provide new insights into understanding the mechanisms of toxicity of MPs to different organisms.

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