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Fluorescence Quenching SERS Detection: a 2D MoS2 Platform Modified with a Large π‐Conjugated Organic Molecule for Bacterial Detection

Laser & Photonics Review 2025 1 citation ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Boyao Lyu, Yongkang Lyu, Liqi Ma, Muhammad Saleem, Abdur Rahim, Mingyue Li, Xiaoyu Zhang, Muhammad Noaman Zahid, Mei Liu

Summary

Despite its title referencing SERS detection, this paper studies a specialized sensor material for detecting bacteria in very low concentrations using surface-enhanced Raman spectroscopy — not microplastic pollution. It examines a MoS2-based heterostructure that improves detection sensitivity by suppressing fluorescence interference, and is not relevant to microplastics or human health.

Polymers

Abstract Surface‐enhanced Raman spectroscopy (SERS) has emerged as a powerful tool for biological detection and analysis. However, it is confronted with challenges from fluorescence interference. This study develops a CuPc/MoS₂ heterostructure with S‐scheme junction through interfacial band alignment. The SERS evaluation of methylene blue (MB) demonstrated a detection limit as low as 10 −10 M, which is 23.37‐fold higher than that of the pristine components. Meanwhile, a fluorescence quenching fraction of 0.925 is achieved by non‐radiative charge recombination, effectively addressing the issue of fluorescence interference and achieving a significant enhancement of SERS signals through the fluorescence quenching of MB molecules. The substrate demonstrates a detection sensitivity of up to 0.611 CFU mL −1 against Escherichia coli (E. coli). Notably, the platform successfully monitored polystyrene nanoplastic (PS‐NP)‐bacteria interactions, revealing size‐dependent membrane disruption mechanisms through flavin adenine dinucleotide (FAD) signal variations. This study establishes a development for non‐metallic SERS substrates in environmental monitoring and nanotoxicology research.

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