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Analyses of microplastic release and surface changes in materials used for intraoral devices subjected to sonication-induced aging
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This planned lab study will test three dental materials, milled acrylic resin, 3D-printed resin, and thermoformed orthodontic aligners, by soaking samples in artificial saliva and aging them with sonication. Researchers will measure microplastic particles released over 14 days and surface roughness changes using microscopy techniques.
Objetivos: Evaluar la liberación de microplásticos (MPs) de distintos materiales poliméricos utilizados en dispositivos intraorales y analizar su relación con la degradación y rugosidad superficial inducidas por un método de envejecimiento acelerado mediante sonicación. Metodología: Se evaluarán tres materiales poliméricos: resina acrílica fresada, resina impresa en 3D y alineador ortodóntico termoformado. Se confeccionarán especímenes estandarizados de cada material, los cuales serán sumergidos en saliva artificial a 37°C y sometidos a un protocolo de envejecimiento acelerado basado en sesiones diarias de sonicación, con recolección del medio a las 24 horas, 7 y 14 días. Se incluirá un grupo control para cada material, mantenido en saliva artificial a 37°C sin sonicación. Los MPs liberados al medio serán analizados mediante Microscopía Electrónica de Barrido (MEB) para determinar tamaño y morfología de las partículas y mediante microscopía de fluorescencia con tinción Nile Red en microscopio holográfico 3D (Nanolive) para corroborar la presencia de micropasticos y analizar su morfologia. Adicionalmente, se evaluará la rugosidad superficial de los especímenes antes y después del envejecimiento. Resultados Esperados: Se espera generar evidencia preliminar sobre la utilidad de este método novedoso de envejecimiento por sonicación, determinando la liberación de MPs desde los materiales evaluados y su relación con los cambios superficiales asociados. Se anticipa identificar diferencias en cantidad, tamaño y morfología de las partículas, así como en la rugosidad superficial entre los distintos materiales tras el protocolo de sonicación.
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