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Label-FreeQuantification of Nanoplastic–CellMembrane Interaction by Single Cell Deformation Plasmonic Imaging
Summary
Researchers developed a label-free quantitative method called Single Cell Deformation Plasmonic Imaging to study nanoplastic interactions with cell membranes, enabling precise measurement of how nanoplastic particles disrupt cellular functions at the membrane level.
Nanoplastics are a growing environmental concern due to their potential to disrupt cellular functions. Understanding how these particles interact with cell membranes is crucial for assessing their biological effects. In this study, we present a label-free, quantitative methodSingle Cell Deformation Plasmonic Imaging (SCDPI)to measure real-time membrane interaction dynamics at the single-cell level. By examining both fixed and live cells, we characterized the binding behaviors of nanoplastics with varying sizes, surface chemistries, and materials. Our findings show that nanoplastic binding induces cell membrane deformation ranging from a few to tens of nanometers, depending on nanoplastic type and concentration (0–250 μg/mL), influencing membrane–surface interactions. This work provides new mechanistic insights into nanoplastic–cell interactions, demonstrating the potential of SCDPI as a powerful tool for evaluating the cellular impacts of environmental pollutants.
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