We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Refined analysis of microplastics in endemic fish species from Lake Victoria using µ-FTIR and pyrolysis GC-MS
Summary
Scientists found tiny plastic bits, like nylon and polypropylene (common in clothing, packaging, and fishing gear), inside fish from Lake Victoria, an important food source for millions of people in East Africa. Using advanced detection methods, they confirmed these plastics were present at measurable levels in the fish's digestive tracts, though not every fish species tested showed contamination. This matters because eating fish that haven't had their guts removed could mean consuming these plastic particles, and better detection methods like the one used here help scientists more accurately track how much plastic pollution is making its way into our food.
Pyrolysis-gas chromatography-mass spectrometry (Pyr-GC-MS) has evolved into one of the most powerful methods for microplastics (MPs) analysis. However, analytical challenges are still encountered whenever Pyr-GC-MS is applied to matrices with high organic matter, protein and lipid contents. In this study, a workflow integrating stereomicroscopy, micro-Fourier transform infrared (µ-FTIR) spectroscopy and Pyr-GC-MS was extended to analyse MPs in three endemic fish species (Protopterus aethiopicus, Rastrineobola argentea and Synodontis victoriae) from Lake Victoria. Stereomicroscopic analysis only detected MPs in the gastrointestinal tracts of P. aethiopicus and S. victoriae, with the highest mean particle numbers being 16.0 ± 7.8 and 14.7 ± 6.7 items/fish taxon, respectively. No MPs were found in whole samples of R. argentea. The microparticles were mostly blue and brown 0.3-1.9-mm fragments and filaments, which µ-FTIR analysis confirmed to be composed of nylon 66, nylon 6/66, nylon 6/10, nylon 11, polyethylene and polypropylene. Retention time locking and post-column backflush considerably reduced the cycle time and increased stability of the Pyr-GC-MS method. Nylon 66 (243 µg/g in S. victoriae), nylon 6 (0.24-96 µg/g), polypropylene (6.4 µg/g in P. aethiopicus), poly(ethylene terephthalate) (1.2-107 µg/g) and styrene-butadiene rubber (8.5-34.8 µg/g) were quantified. Polyethylene and poly(methyl methacrylate) were detected below their limits of quantification. To prevent false positive detection of polyethylene in the lipid-rich tissues, the evaluation focussed on C21-α,ω-alkene as a marker. Polypropylene was not detected in some samples, as it formed several discrete propylene oligomers during pyrolysis, which suppressed the yield of its target quantification marker (2,4-dimethyl-1-heptene).