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Intestinal toxicity of functionalized polystyrene nanoplastics in NOD2 models of Crohn's disease susceptibility
Original title: Intestinal toxicity of functionalized polystyrene nanoplastics in NOD2 models of Crohn’s disease susceptibility
Summary
Tiny plastic particles from everyday products—like those found in food packaging and water—can damage the gut lining, reduce protective mucus, and trigger inflammation-related changes, according to new research using mice and cell models. This matters most for people genetically predisposed to Crohn's disease, since the study found their gut cells reacted similarly (or in some cases showed unique responses) to these nanoplastics compared to healthy models, suggesting people with this genetic risk factor may be more vulnerable to plastic exposure's effects on gut health. More research is needed, but this is an early signal that not everyone may be equally affected by the micropl
The daily use of plastic products leads to exposure of populations via ingestion, which may affect intestinal health. While a wealth of studies has documented the impact of micro- and nanoplastics (MNPLs) on models representing healthy human intestine, less is known about their impact on sensitive populations, particularly those at risk of developing inflammatory bowel diseases. This study explored the intestinal response to carboxylated and aminated polystyrene particles (50–500 nm PS-COOH and 50 nm PS-NH 2 ) in vivo and in vitro in models of genetic susceptibility to Crohn’s disease. These models included nucleotide-binding oligomerization domain-2 wild-type and knock-out mice (Nod2 WT and Nod2 KO , respectively) and co-cultures of Caco-2 and HT29-MTX cells, with Caco2 cells stably expressing either wild-type NOD2 or the NOD2 1007fs variant, which is strongly associated with Crohn’s disease. In vitro, the co-cultures were used either in a non-differentiated state ( i.e. , 24 h post-seeding) or after differentiation into a tight, mucus-secreting epithelium. Results showed that in vivo , single oral administration of PS-COOH particles impaired the paracellular permeability of the ileum of Nod2 WT mice. Both PS-COOH and PS-NH 2 reduced the number of goblet cells in Nod2 WT and Nod2 KO mice as well as Muc4 secretion in Nod2 WT mice, and PS-COOH reduced Muc4 secretion in Nod2 KO mice. Single oral administration of PS-COOH or PS-NH 2 altered mRNA expression of genes involved in the production of antimicrobial peptide, inflammation, mucin secretion and endoplasmic reticulum stress pathways, in both Nod2 WT and Nod2 KO mice. In vitro, PS-COOH showed no impact in Caco2-NOD2 1007fs /HT29-MTX and in Caco2-NOD2 WT /HT29-MTX, whereas PS-NH 2 induced a similar toxic response in both cell systems. The intensity of this response depended on the cells’ differentiation status and on the mode of exposure, i.e., acute versus repeated. Overall, this study explored, for the first time, the response of models of genetic susceptibility to Crohn’s disease to exposure to PS-COOH and PS-NH 2 nanoplastics (NPLs). The obtained data provide new insight into how populations at risk of developing CD respond to PS-COOH and PS-NH 2 NPLs, i.e., new knowledge related to the impact of NPLs on this vulnerable population.