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Microplastic-induced gut dysbiosis and metabolic alterations in juvenile European seabass (Dicentrarchus labrax): A multi-omics approach

Marine Pollution Bulletin 2026 2 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Yossra Missaoui, Massimo Venditti, Leilei Zhang, Filippo Vaccaric, Mohamed Rida Abelouah, Siwar Abouda, Sonia Gaaieda, Edoardo Puglisi, Luigi Lucini, Sergio Minnucci, Mohamed Bannı

Summary

Scientists found that when sea bass (a fish we eat) were exposed to real-world microplastic particles collected from the environment, it triggered inflammation, cell damage, and disrupted energy metabolism in their gut, while also killing off beneficial gut bacteria and letting pollution-tolerant bacteria take over. While this study was done in fish, not humans, it matters because it shows that microplastics—which are now found throughout our food chain and environment—can meaningfully disrupt gut health and the microbiome, raising questions about similar effects in people who consume seafood or are otherwise exposed to these particles.

Polymers
Body Systems

Environmental microplastics (MPs) are increasingly recognized as emerging contaminants with the potential to disrupt intestinal homeostasis in marine organisms. However, most experimental evidence is based on pristine particles rather than environmentally weathered forms. This study investigated the intestinal effects of environmentally derived microplastics (EMPs) in juvenile European seabass (Dicentrarchus labrax) using an integrated multi-omics approach. Fish were exposed for five days to two concentrations of EMPs (0.5 and 1 mg/kg of feed), followed by analyses combining histological, transcriptomic, metabolomic, and metagenomic endpoints. EMP exposure led to significant particle accumulation in gut tissues, predominantly consisting of small polyethylene fragments. Gene expression and immunofluorescence analyses revealed activation of p53 and Caspase-3 mediated apoptosis together with NF-κB and IL-6 driven inflammatory signalling, indicating concurrent oxidative and immune stress. Untargeted metabolomics identified marked alterations in lipid metabolism, redox regulation, and amino acid turnover, consistent with mitochondrial dysfunction and impaired energy homeostasis. Parallel metagenomic profiling revealed subtle but coherent shifts in gut bacterial communities, with enrichment of pollutant-tolerant taxa such as Acidovorax and Halioglobus and reduction of beneficial commensals such as Ligilactobacillus. Multi-omics data integration demonstrated a coordinated restructuring of microbial and metabolic networks underlying host physiological stress. Collectively, these findings highlight the intestine as a primary target of microplastic toxicity and provide mechanistic insight into early biological responses to environmentally realistic microplastic exposure in marine fish.

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