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Explainable deep-learning detection of microplastic fibers via polarization-resolved holographic microscopy

Optics Express 2026 1 citation ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Jan Appel, Marika Valentino, Lisa Miccio, Vittorio Bianco, Raffaella Mossotti, Giulia Dalla Fontana, Miroslav Ježek, Pietro Ferraro, Jaromír Běhal

Summary

Scientists have developed a smart imaging tool that shines specially patterned light on tiny fibers to tell plastic microfibers apart from natural ones like cotton and wool, with about 97% accuracy. This matters because tracking exactly how much plastic pollution is in our water, food, and air is a key step toward understanding how microplastics might affect our health — and this faster, more reliable detection method could help researchers monitor that exposure more effectively.

Body Systems

Reliable identification of microplastic fibers is crucial for environmental monitoring but remains analytically challenging. We report the first explainable deep-learning framework for classifying microplastic and natural microfibers using exclusively polarization-based features obtained from polarization-resolved digital holographic microscopy. From multiplexed holograms, the complex Jones matrix of each fiber was reconstructed to extract polarization eigen-parameters describing optical anisotropy. Statistical descriptors of nine polarization characteristics formed a 72-dimensional feature vector for a total of 296 fibers spanning six material classes, including polyamide 6, polyethylene terephthalate, polyamide 6.6, polypropylene, cotton, and wool. The designed fully-connected deep neural network achieved an accuracy of 96.7 % on the validation data, surpassing that of common machine-learning classifiers. Explainable artificial intelligence analysis with Shapley additive explanations identified eigenvalue-ratio quantities as dominant predictors, revealing the physical basis for classification. An additional reduced-feature model with the preserved architecture, exploiting only these most significant eigenvalue-based characteristics, retained high accuracy (93.3 %), thereby confirming their dominant role while still outperforming common machine-learning classifiers. These results establish polarization-based features as distinctive optical fingerprints and demonstrate an explainable deep-learning approach for automated microplastic fiber identification.

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