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Polystyrene micro- and nanoplastics exposure during pregnancy compromises offspring skin barrier function via interference with PPAR-mediated lipid metabolism

Journal of Hazardous Materials Advances 2026
Qingwen Zeng, Ting Li, Jingchao Zhou, Zhongyin Liang, Wei Dong, Qianqian Sun, Shiyi Xiong, Xing Li, Jing Li, Bin Du, Guangquan Chen

Summary

When pregnant mice drank water containing micro- and nanoplastics, their newborn pups developed weaker, leakier skin that lost more moisture than normal — the plastics disrupted key fat molecules the skin needs to form a healthy protective barrier. This matters because it suggests that plastic pollution exposure during pregnancy could affect a baby's skin development before birth, though more research is needed to confirm whether this happens in humans too.

Polymers
Body Systems
Models
Study Type In vitro

The ubiquitous presence of micro- and nanoplastics (PS-MNPs) in the environment has raised significant concerns regarding their potential developmental toxicity. However, the impact of maternal polystyrene(PS) - MNP exposure on the skin barrier function of offspring remains unknown. In this study, pregnant mice were exposed to PS-MPs and PS-NPs (10 mg/L) via drinking water during gestation. We assessed the neonatal (P0) skin development through histological analysis and trans-epidermal water loss (TEWL) measurements. Multi-omics approaches were employed to identify toxic mechanisms. HaCaT cells were employed as an in vitro model to investigate cellular uptake, localization, and cytotoxicity of PS-MNPs. Maternal exposure to both PS-MPs and PS-NPs significantly impaired epidermal barrier development in P0 offspring. At an exposure level of 10 mg/L, TEWL values were significantly elevated, accompanied by a compensatory increase in epidermal thickness. Multi-omics analysis revealed that PS-MNP exposure suppressed the PPARα -mediated lipid metabolic pathway, leading to a systemic imbalance in lipid homeostasis within the skin tissue. Interestingly, PS-MPs specifically triggered dysregulation in DNA replication and cell cycle-related signaling. In vitro assays demonstrated that PS-NPs could penetrate HaCaT cells and accumulate in lysosomes and cell membranes, exerting dose-dependent cytotoxicity. Conversely, PS-MPs primarily resided in the intercellular spaces and exhibited lower toxicity. Our findings demonstrate that maternal PS-MNP exposure compromises offspring skin barrier integrity by interfering with PPARα -mediated lipid metabolism. This study provides critical evidence for the health risks of maternal MNPs exposure on neonatal skin development, and identifies potential biomarkers for MNPs-induced cutaneous toxicity.

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