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Evaluating the in Vitro Toxicity of 4-MBC and Microplastics: The Role of PE and PVC as Carriers

Journal of Polymers and the Environment 2025
Nataša Milić, Maja Milanović, Jovana Drljača Lero, Aleksandra Popović, Sanja Vasiljević, Tajana Simetić, Aleksandra Tubić

Summary

Researchers evaluated the cytotoxicity of the UV filter 4-MBC sorbed onto polyethylene and PVC microplastics in human embryonic lung fibroblast cells, finding that PE-bound 4-MBC produced a synergistic toxic interaction while PVC combinations showed additive effects. The results demonstrate that microplastics acting as chemical carriers can amplify the health hazards of co-pollutants beyond what either contaminant poses alone.

Polymers
Study Type In vitro

UV filter, 4-methylbenzylidene camphor (4-MBC) is identified as endocrine disrupting chemical that is frequently detected in the aquatic environment. Due to its hydrophobic nature and stability under UV radiation, the concomitant presence of both 4-MBC and microplastics (MPs) enlightens the possible impacts of their combined effects on human health. Hence, the aim of this research was to evaluate the adsorption capacity of 4-MBC in the presence of two commonly encountered types of MPs in the environment: polyvinyl chloride (PVC), and polyethylene (PE). Having in mind that inhalation is one of the primary exposure routes to MPs, the human embryonic lung fibroblast cell line (MRC-5) was used to study the individual cytotoxicity of 4-MBC, PVC, and PE, as well as their sorbed samples. The equilibrium adsorption capacity of 4-MBC on PE was higher compared to PVC. The combined effects of PVC + 4-MBC as well as PE + 4-MBC exerted non-linear dose-response dynamics. The enhanced cytotoxicity of adsorbed samples was detected. Moreover, more pronounced cytotoxic effect was observed in cells treated with PE + 4-MBC. The cooperative index showed an additive effect for 4-MBC and PVC, while 4-MBC and PE exhibited a synergistic interaction. Based on the obtained results PE can sorb and concentrate a higher amount of 4-MBC compared to PVC.

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