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A Dual-Laser Raman Strategy for Fast and Direct Detection and Quantification of Microplastics in Water
Summary
Researchers developed a dual-laser Raman strategy that simultaneously captures backscattering and transmission signals from two 532 nm lasers to quantify microplastics directly in water without filtration or drying, achieving roughly 1.5- to 3-fold greater signal stability and accurate real-time particle counting at 0.1-second temporal resolution.
Reliable quantification of microplastics in water remains challenging because most Raman-based methods require filtration, drying, or complex flow systems, which can lead to particle loss and signal instability. Here, we propose a simple dual-laser Raman strategy for the direct, real-time quantification of microplastics in water without pretreatment. By simultaneously integrating backscattering and transmission geometries using two identical 532 nm lasers, spatial variations in Raman scattering cross-sections, arising from particle motion and focal depth fluctuations, are effectively mitigated. The dual-laser configuration enhances Raman intensity by approximately 1.5-fold compared with backscattering and threefold compared with transmission alone (p < 0.001), enabling robust real-time detection with a temporal resolution of 0.1 s. Accurate particle counting is demonstrated using polystyrene (PS) standard beads and further validated for polyamide 6 (PA6) and polyvinyl chloride (PVC) particles with irregular morphologies and broad size distributions, with no false-positive events observed. By prioritizing simplicity and quantitative reliability over ultimate size resolution, the proposed strategy provides a practical approach for routine monitoring of microplastics in drinking water and industrial aqueous systems.