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10.51847/9wOM6VobvV
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This study aimed to isolate polyethylene-degrading Pseudomonas bacteria and clone the alk-B gene responsible for alkane breakdown, using both direct culture and pre-enrichment methods to identify strains with potential for accelerating biological degradation of high-density plastic waste.
Today, high-density plastics such as polyethylene are recognized as one of the major pollutants of the environment by the Global Environment Organization. This study has been designed to isolate bacterial strains of Pseudomonas with polyethylene degradation power and to clone the alk-B gene to be used in the future to expedite the biological degradation of plastic waste. To isolate the polyethylene degrading bacteria, two culture methods were used, direct culture and pre-enriched medium. Bacteri
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