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Isolation and Characterisation of Polyhydroxybutyrate (PHB) Producing Bacteria for Sustainable Bioplastic Development
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This paper isolates and characterizes bacterial strains capable of synthesizing polyhydroxybutyrate (PHB), a naturally occurring bioplastic stored as an energy reserve by certain microorganisms. Researchers identified promising bacterial candidates and assessed their PHB yield and production conditions. These findings support ongoing efforts to develop microbially produced bioplastics as biodegradable alternatives to conventional petroleum-based polymers.
Polyhydroxybutyrate (PHB), a biodegradable biopolymer produced by certain bacteria, is one of the sustainable alternatives being sought after due to the growing environmental burden posed by traditional plastics. In this study, environmental samples were taken from Dumas Beach and Kholwad College Garden in South Gujarat in order to isolate, identify, and optimize PHB-producing bacterial strains. Using the Carbol Fuchsin, Sudan Black B, and Nile Blue A staining methods, a total of 25 bacterial isolates were acquired and tested. Based on morphological, biochemical, and staining investigations, two strains—Bacillus spp. and Pseudomonas spp.—were determined to be effective PHB producers. Molasses, leftover cooking oil, orange and potato peels, and other low-cost carbon sources were assessed for PHB production. The largest PHB concentration was found in potato peels, where Pseudomonas spp. could reach 89%. PHB yield was considerably increased by a pH of 7, a temperature of 37°C, and shaking conditions, according to the results of production parameter optimization. Fourier Transform Infrared Spectroscopy (FTIR), which showed distinctive peaks of ester carbonyl and C–H functional groups, verified that the isolated polymer was PHB. This research highlights the potential of using regionally isolated bacteria and agro-waste materials for low-cost, eco-friendly PHB production. These findings support sustainable bioplastic development and offer promising avenues for industrial-scale biopolymer synthesis using locally sourced resources.
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Researchers isolated PHB-producing bacteria from soil samples and characterized the extracted polyhydroxybutyrate bioplastic using FTIR spectroscopy and electron microscopy, with the top-performing isolate achieving a 52% PHB yield and successfully fabricating biodegradable nanofibers as a potential eco-friendly alternative to petroleum-derived plastics.
Characterization and Process Optimization for Enhanced Production of Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Bacillus flexus Isolated from Municipal Solid Waste Landfill Site
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Researchers isolated Bacillus flexus bacteria from a municipal landfill and optimized conditions for producing polyhydroxybutyrate (PHB), a biodegradable bioplastic, finding that stress conditions like nitrogen limitation significantly boosted polymer accumulation and yield.
Screening, identification and characterisation of Polyhydroxybutyrate producing bacteria from garden soil
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Researchers screened soil bacteria for their ability to produce polyhydroxybutyrate (PHB), a biodegradable biopolymer that can replace petroleum-based plastics. Five promising bacterial isolates were identified and characterized, with production conditions optimized. Scaling up microbial PHB production from inexpensive soil microbes is an important step toward making biodegradable plastic alternatives cost-competitive with conventional plastics.
Characterization of Polyhydroxybutyrate Producing Bacterium Isolated from Sewage Sample
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Researchers identified and characterized bacterial strains isolated from sewage samples that are capable of producing polyhydroxybutyrate (PHB), a biodegradable thermoplastic. The study screens natural microbial communities for strains useful in sustainable bio-based plastic production.
Phenotypic Screening and VITEK®2-Based Identification of Polyhydroxybutyrate (PHB)- Producing Bacteria isolated from Agro-Industrial Waste Sites in Iraq
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Researchers isolated 15 bacterial strains from agro-industrial waste sites in Iraq and identified six capable of accumulating polyhydroxybutyrate (PHB), a biodegradable bioplastic, including Enterobacter cloacae and Escherichia coli, demonstrating that industrial waste environments are promising reservoirs for bacteria that can support sustainable bioplastic production.
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