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ISOLATION AND CHARACTERISATION OF MICROPLASTIC ABUNDANCE IN Lates calcarifer FROM SETIU WETLANDS, MALAYSIA
Summary
This study successfully isolated and characterized microplastics from wild and cage-cultured Asian sea bass in Malaysian wetlands, finding microplastics in both wild and farmed fish. The results confirm that aquaculture fish are also exposed to environmental microplastic contamination, raising questions for food safety.
The presence of microplastics (<1 mm) in wild and cage-cultured Asian sea bass (Lates calcarifer) was successfully studied.Fish samples were collected from Setiu Wetlands in October 2016.Microplastics were isolated from fish samples using the alkaline solution method (10 M of NaOH solution).Microplastics were sorted visually according to their shapes and colours after being observed under dissecting microscope.A total of 4,498 pieces of microplastics were identified and threadlike shape was the most abundant microplastic particles found during this study.The identification of functional groups in the composition of microplastics was achieved using Fourier transform infrared (FTIR) spectroscopy.Microplastics, with the presence of strong peaks at ʋ=3342 cm -1 (N-H str), 1510 cm -1 (N-H bend) and ʋ=1706 cm -1 (C=O str) are associated with the materials consist of polyamide.Additionally, peaks related to the polyvinyl alcohol groups are recorded at ʋ= 3321 -4323 cm -1 (O-H str), 1706 cm -1 (C=O str), 1219 cm -1 (O-H wag) and ʋ=1028 -1128 cm -1 (C-O str).The abundance levels of microplastics ingested by wild and cage-cultured fishes are statistically significantly different, p <0.05.