Effect of Microplastic Amendment to Food on Diet Assimilation Efficiencies of PCBs by Fish

Diet assimilation efficiencies (AEs) of polychlorinated biphenyls (PCBs) absorbed to microplastics and food were determined in goldfish ( Carassius auratus). Microplastics were spiked with 14 environmentally rare PCBs and incorporated into fish pellets previously spiked with a technical PCB mixture (Aroclor 1254). Five diet treatments were created having microplastic contents of 0, 5, 10, 15, 20, and 25% and fed to fish within 24 h of the diet creation. Fish from each treatment were fed a microplastic amended food pellet and PCB AEs were determined by mass balance. Microplastic-associated PCBs had lower AEs (geomean 13.36%) compared to food matrix-associated PCBs (geomean 51.64%). There were interactions between PCB AEs and the microplastic content of the diet. PCBs affiliated with microplastics became more bioavailable with increasing microplastic content of food while food matrix-associated PCB bioavailability declined when microplastic contents exceeded 5%. Despite controlling for microplastic-food contact time, there was some evidence for redistribution of lower KOW food matrix-associated PCBs onto microplastics causing a decrease in their AE relative to nonplastic and low plastic containing diets. The low bioavailability of microplastic-associated PCBs observed in the present study provides further support to indicate that microplastics are unlikely to increase POPs bioaccumulation by fish in aquatic systems.