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Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography

Environment International 2019 131 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 50 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Yuyi Yang Wenjuan Song, Yuyi Yang Yuyi Yang Wenjuan Song, Yuyi Yang Wenzhi Liu, Hui Lin, Wenzhi Liu, Wenzhi Liu, Zan Li, Linna Du, Linna Du, Zan Li, Yuyi Yang Yuyi Yang Zan Li, Wenjuan Song, Wenzhi Liu, Wenjuan Song, Wenjuan Song, Wenjuan Song, Hui Lin, Hui Lin, Hui Lin, Hui Lin, Yuyi Yang Linna Du, Linna Du, Linna Du, Hui Lin, Wenzhi Liu, Hui Lin, Wenzhi Liu, Wenzhi Liu, Yuyi Yang Linna Du, Linna Du, Linna Du, Chen Ye, Chen Ye, Yuyi Yang Hui Lin, Yuyi Yang Hui Lin, Wenzhi Liu, Hui Lin, Linna Du, Chen Ye, Hui Lin, Yuyi Yang Hui Lin, Linna Du, Hui Lin, Wenzhi Liu, Yuyi Yang Wenzhi Liu, Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Zan Li, Yuyi Yang Wenzhi Liu, Wenzhi Liu, Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Yuyi Yang Hui Lin, Hui Lin, Hui Lin, Hui Lin, Hui Lin, Hui Lin, Zan Li, Zan Li, Bo Zhao, Yuyi Yang Wenzhi Liu, Wenzhi Liu, Wenzhi Liu, Hui Lin, Wenzhi Liu, Yuyi Yang Hui Lin, Yuyi Yang Yuyi Yang Hui Lin, Hui Lin, Hui Lin, Yuyi Yang Danli Deng, Zan Li, Danli Deng, Zan Li, Yuyi Yang Yongtai Pan, Hui Lin, Yongtai Pan, Hui Lin, Yuyi Yang Zan Li, Xinhua Cao, Xinhua Cao, Hui Lin, Yuyi Yang

Summary

A global survey of 122 lake and seawater metagenomic datasets found that antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were significantly more abundant in lake water than seawater, with multidrug resistance and bacitracin resistance as the most prevalent ARGs. The study provides a large-scale baseline for resistance gene abundance in aquatic environments and its variation with geography and stratification.

Study Type Environmental

A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34 × 100 and 1.22 × 10-3-1.98 × 10-1 copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multi-metal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater.

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