Evaluation of the hazard of irregularly-shaped co-polyamide microplastics on the freshwater non-biting midge Chironomus riparius through its life cycle

Plastics pollution is increasingly attracting societal and political attention. However, despite extensive research effort recently dedicated to the hazard of plastics in the environment, the data obtained are often redundant and essential knowledge gaps exist: available freshwater ecotoxicity data mostly concern Daphnia magna and are derived from acute exposure to spherical particles. In this paper, we address this gap by exploring the biological effects of irregularly-shaped co-polyamide (PA, 10-180 μm) on Chironomus riparius - a very versatile organism that during its life-stages inhabits both sediment and water column - relevant compartments for microplastics (MP) pollution. C. riparius represents an important part of the freshwater food chain and is also a standard OECD test organism. Different toxicity endpoints along the life cycle of C. riparius (28 days) were used as described in OECD 218: emergence, time to emergence, sex ratio of imagoes and the number of egg clutches per female. Chironomid larvae were exposed to 100 mg PA kg (i.e., 10,100 particles kg) sediment throughout. Soluble Zn-salt (1 mg Zn L) was used as a positive control and as a co-pollutant in combination with PA. We demonstrated that the tested concentrations of PA and Zn alone as well in combination showed no adverse effects for C. riparius in chronic exposures. 100 mg PA kg also did not affect the life cycle traits of the offspring of PA-exposed parent Chironomids. The data obtained will be useful for environmental risk assessment of PA when actual environmental concentrations of PA will be available.