We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Profiling of microRNAs and mRNAs in marine mussel Mytilus galloprovincialis
Summary
Researchers used deep sequencing to profile microRNAs in the Mediterranean blue mussel Mytilus galloprovincialis, identifying 137 miRNA sequences — 104 conserved across species and 33 potentially novel — providing a genomic baseline for studying how this ecologically important species responds to environmental stressors.
MicroRNAs (miRNAs) are a class of noncoding RNA molecules containing 18-24 nucleotides, and those with conserved structures are able to regulate the expression of eukaryotic genes by inhibition or enhancement of mRNA translation. However, miRNAs of the blue mussel, Mytilus galloprovincialis have not been reported. M. galloprovincialis is a primary species distributed along coastal zones worldwide. To reveal the repertoire of miRNAs in M. galloprovincialis, we constructed small RNA libraries prepared from three different mussels, which were then sequenced by Solexa deep sequencing technology. A total of 32,836,817, 33,359,113 and 33,093,562 clean reads from the tissues of the three M. galloprovincialis were obtained. Based on sequence similarities and hairpin structure predictions, 137 M. galloprovincialis miRNAs (mg-miRNA) were identified. Among the mg-miRNAs, 104 were conserved across species, whereas 33 might be novel and specific for M. galloprovincialis. Some of the mg-miRNAs, such as let-7 and the miR-100 family are playing key roles in many metabolic pathways and are worthy of further study. By performing a whole genome-scale characterization of mg-miRNAs and proposing their potential functions, these results provide a foundation for understanding the biological processes of the blue mussel, M. galloprovincialis.
Sign in to start a discussion.
More Papers Like This
The native mussel Mytilus chilensis genome reveals adaptative molecular signatures facing the marine environment
Researchers sequenced and analyzed the genome of the native Chilean blue mussel Mytilus chilensis, identifying adaptive molecular signatures that reflect the species' responses to diverse marine environmental conditions, with implications for understanding resilience in an aquaculture species reliant on wild seed collection.
Responses of microRNA in digestive glands of mussel Mytilus galloprovincialis exposed to polystyrene nanoplastics
Polystyrene nanoplastics triggered significant microRNA expression changes in the digestive glands of the mussel Mytilus galloprovincialis, with altered miRNAs involved in immune regulation, apoptosis, and stress response pathways. The findings indicate that microRNA-mediated gene regulation is an important molecular mechanism of nanoplastic toxicity in marine bivalves.
Identification of microRNA-mRNA regulatory network associated with microplastic exposure in Mytilus galloprovincialis
Scientists identified specific microRNA-mRNA regulatory networks in Mediterranean mussels that are altered by microplastic exposure, revealing how plastic pollution affects gene regulation at the molecular level. The study found that microplastics disrupt biological pathways related to development, growth, and reproduction in these filter-feeding organisms. Since mussels are widely consumed as seafood, the findings also raise concerns about microplastics entering the human food chain.
Chromosome-Level Genome Assembly of the Blue Mussel Mytilus chilensis Reveals Molecular Signatures Facing the Marine Environment
Scientists assembled the first chromosome-level genome of the Chilean blue mussel, an important aquaculture species in South America. The genome revealed genetic adaptations related to immune defense, stress response, and shell formation that help the mussels survive in challenging marine environments. This genomic resource will support breeding programs and help researchers understand how mussels respond to environmental stressors including pollution.
Hypoxia in the Blue Mussel Mytilus chilensis Induces Transcriptome Shift Associated with Endoplasmic Reticulum Stress, Metabolism, and Immune Response.
Scientists used gene expression analysis to study how low-oxygen conditions affect the blue mussel Mytilus chilensis at the molecular level. Researchers found that hypoxia triggered stress responses in the mussels' gills, digestive glands, and muscles, including cellular stress pathways, metabolic shifts, and immune changes. The study highlights how climate-driven oxygen depletion in coastal waters can stress economically important shellfish species.