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Toxicological effects of graphene on mussel Mytilus galloprovincialis hemocytes after individual and combined exposure with triphenyl phosphate

Marine Pollution Bulletin 2020 25 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Xiangjing Meng, Fei Li, Xiaoqing Wang, Jialin Liu, Chenglong Ji, Huifeng Wu, Huifeng Wu

Summary

Researchers exposed mussel immune cells to graphene nanoparticles and the flame retardant triphenyl phosphate, finding that each contaminant alone induced oxidative stress and DNA damage, but their combination paradoxically reduced these harmful effects by activating cell-survival gene pathways.

Graphene nanoparticles are increasingly released into the aquatic environment with the growth of production. However, there are rare investigations focusing on the interaction of nanoparticles with other contaminants. Triphenyl phosphate (TPP) is a frequently detected organophosphate flame retardant in the environment. This study aimed to assess the joint effects of graphene and TPP on Mytilus galloprovincialis hemocytes. Oxidative stress could be induced by graphene and TPP in mussel hemocytes, which could further cause apoptosis, DNA damage and decrease in the lysosomal membrane stability (LMS). Moreover, hemocytes could internalize graphene, thereby resulting in oxidative stress. The oxidative stress and DNA damage in hemocytes were increased in the graphene-exposed group, but significantly reduced after combined exposure of graphene and TPP. The up-regulated genes, including NF-κB, Bcl-2 and Ras, were mainly associated with reduced apoptosis and DNA damage after co-exposure to graphene and TPP.

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