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Optical imaging methods for label free detection of microplastics in cells, tissues and environmental organisms (Conference Presentation)
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This study reviews label-free optical imaging methods for detecting microplastics in cells and tissues, including coherent Raman scattering, stimulated emission depletion, and autofluorescence techniques. Researchers found that these approaches enable visualization of plastic particles in biological samples without chemical staining, improving throughput and reducing artifacts.
Microplastic particles are a major environmental pollution problem. Challenges in microplastics detection are the broad size range, chemical heterogeneity and low concentrations in liquids. Label free optical imaging methods for the detection and particle type identification were tested and compared. Quantitative Phase Imaging and holographic tomography were used to study cell response and cell uptake of microplastic particles. Optical Coherence Tomography could analyze larger organisms as Daphnia magna for microplastics uptake. Imaging methods combining particle localization with a chemical identification as RAMAN and mid IR spectroscopy could clearly identify different particle types and even detect specific microplastic chemistries inside cells.
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