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Multiline illumination Raman microscopy for rapid cell imaging
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Researchers developed a multiline illumination approach for Raman microscopy that dramatically accelerates image acquisition compared to conventional point-scanning methods. The technique enables faster chemical imaging of biological samples with potential applications in medical diagnostics and microplastic detection.
Acceleration of image acquisition rate in Raman microscopy has been required to fully utilize its analytical advantages for biological/medical applications. By introducing the multiple line illumination and parallel spectral detection capability, image acquisition rate in the Raman microscope was improved < 104 times, compared with the conventional confocal Raman. High-resolution spontaneous Raman imaging of cells/tissues was thus enabled with an image acquisition time of a few minutes. Subsequent high-throughput Raman imaging-based analyses were also performed, including multiplex Raman tag imaging, cell classification, microplastic detection.
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