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Article ? AI-assigned paper type based on the abstract. Classification may not be perfect — flag errors using the feedback button. Tier 2 ? Original research — experimental, observational, or case-control study. Direct primary evidence. Detection Methods Marine & Wildlife Sign in to save

Evidence of small microplastics (<100 μm) ingestion by Pacific oysters (Crassostrea gigas): A novel method of extraction, purification, and analysis using Micro-FTIR

Marine Pollution Bulletin 2020 60 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Fabiana Corami, Beatrice Rosso, Marco Roman, Marco Picone, Andrea Gambaro, Carlo Barbante

Summary

Researchers developed a novel extraction, purification, and micro-FTIR analysis method to detect small microplastics under 100 micrometers in Pacific oysters (Crassostrea gigas), confirming ingestion of these smaller particles and finding that existing methods routinely miss this size fraction due to inadequate tissue digestion protocols.

Microplastics (MPs) are present in fresh, brackish, or marine waters. Micro- and macroinvertebrates can mistake MPs or small microplastics (SMPs, <100 μm) to be food particles and easily ingest them according to the size of their mouthparts. SMPs may then block the passage of food through the intestinal tract (i.e. hepatopancreas), accumulate within the organism, and enter the food web. Pacific oysters (Crassostrea gigas) are allochthonous filter-feeding bivalve mollusks, which have been introduced in coastal seas around the world in both natural banks and farms. Considering their economic and ecological value, these bivalves have been chosen as a model to study the ingestion of SMPs. A novel method for the extraction and purification of SMPs in bivalves was developed. Quantification and simultaneous polymer identification of SMPs using Micro-FTIR (Fourier Transform Infrared Spectroscopy) were performed, with a limit of detection for the particle size of 5 μm.

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