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Seafood Inspection: Current Issues

Archivio Istituzionale della Ricerca (Universita Degli Studi Di Milano) 2020
Chiara Coppola

Summary

This PhD thesis investigates quality and safety aspects of seafood including lobster stress during commercialization, microbiological contamination, and chemical hazards. The work combines behavioral, haemolymph, microbiological, and chemical analyses with regulatory considerations to provide a multidisciplinary assessment of seafood safety. This is a thesis compilation covering multiple seafood safety topics.

The thesis focuses on quality and safety aspects of different types of seafood by using a multidisciplinary approach, combining different types of analyses (behavioural studies, haemolymph analyses, microbiological and chemical-physical analyses) and regulatory aspects. The thesis consists of a collection of scientific papers and laboratory activities that report the main studies of my PhD research. In the first study the evaluation of the stress level during lobster commercialization was evaluated by analysing eight different haemolymph parameters. The second study faced the potential application of a new Loop-mediated isothermal amplification (LAMP) assay for the detection of Anisakis spp. in seafood products. The third study shows the preliminary results of an ongoing investigation that aims at developing a method suitable for the extraction of microplastics from mussels. The fourth study compared the chemical composition in fish roe products from different species in order to achieve a deeper knowledge of the chemical and microbiological composition of fish roe products collected on the Italian market.

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