Comparative study of three sampling methods for microplastics analysis in seawater

Microplastics, as an emerging pollutant, are widely spread in the oceans. The sampling method is the most basic and important factor affecting the reported microplastic abundance data. Three sampling methods, most commonly used for microplastic collection, including direct filtration with 0.45 μm pore size membrane, 20 μm sieve pre-concentration followed by 0.45 μm filtration and Manta trawling with a 150 μm mesh size net were studied. The results showed that there were orders of magnitude difference in abundance of microplastic across the three methods with 0.45 μm direct filtration yielding 1600.0-4000.0 items/m, 20 μm sieve pre-concentration yielding 10.0-50.0 items/m, and 150 μm trawl net yielding 0.13-0.24 items/m. The polymer types of microplastic collected by the three methods were similar, but polymer proportions were different. PES and rayon dominated in the samples collected by direct filtration and sieve pre-concentration. PES and PP accounted for a higher proportion in the trawling samples. The abundance and polymer types of microplastics had a clear correlation between direct filtration and sieve pre-concentration (p < 0.05). More microplastic shapes were found in trawling samples. The average and median sizes of microplastics increased with increasing pore or mesh size. According to the size fraction and standard deviation of microplastic size in different samples of each method, the stability of method decreased with increasing pore or mesh size. The trawling method had a higher sampling efficiency, but its stability was weaker than the other two methods. Our results suggested that an appropriate method should be selected upon actual sampling condition and available tools during the research process to improve the credibility of the results.