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Nanoplastic sizes and numbers: quantification by single particle tracking
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A sensitive fluorescence video microscopy method combining Nile Red staining with single particle tracking was developed to count and size nanoplastic particles in water. The technique enables quantification of plastic nanoparticles without expensive specialized equipment, making nanoplastic monitoring more accessible.
Easy method to count and size plastic nanoparticles in water. A combination of sensitive fluorescence video microscopy, NileRed staining of plastic particles, and single particle tracking allows for counting and sizing nanoplastics.
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Nanoplastic Sizes and Numbers: Quantification by Single Particle Tracking
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Researchers combined sensitive fluorescence microscopy with single particle tracking to quantify the size and number of nanoplastic particles in dilute suspensions. The method fills a critical gap in the ability to detect and characterize the smallest plastic particles in environmental samples.
Nanoplastic Sizes and Numbers: Quantification by Single Particle Tracking
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Researchers developed single particle tracking to quantify nanoplastic particle sizes and concentrations in water samples. The technique enables direct measurement of nanoplastic number concentrations and size distributions, addressing a critical gap in nanoplastic characterization.
Rapid detection of nanoplastics and small microplastics by Nile-Red staining and flow cytometry
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Researchers developed a rapid method for detecting nanoplastics and small microplastics by combining Nile-Red fluorescent staining with flow cytometry. The technique can quantify plastic particles in the 0.6 to 15 micrometer range in just 90 seconds, which is hundreds of times faster than conventional spectroscopic methods. The approach showed high detection efficiency for polyethylene, polyvinylchloride, and polystyrene, offering a practical tool for environmental nanoplastic monitoring.
Highly efficient Nile red staining for the rapid quantification of microplastic number concentrations using flow cytometry
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Scientists developed an improved method for staining microplastics with a fluorescent dye (Nile red) that embeds the dye inside the plastic particles rather than just coating the surface, resulting in much brighter and more reliable detection. Combined with high-speed flow cytometry, the technique can rapidly count microplastic particles smaller than 10 µm in environmental water samples with recovery rates above 99%. Faster and more accurate counting methods like this are important for scaling up microplastic monitoring across many water sources.
A new method for counting and sizing microplastic particles in water: investigating the presence of microplastics in Iranian bottled water
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Researchers developed a novel method for counting and sizing microplastic particles in bottled water using Nile Red fluorescent staining combined with direct microscopic counting. Testing Iranian bottled water brands revealed widespread microplastic contamination, with the new method providing a faster and more accessible alternative to spectroscopic identification for routine bottled water monitoring.
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