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An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films
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Researchers developed a fast absorbance-based method to assess PET-degrading enzyme (PETase) kinetics and used it to characterize a thermostable engineered variant (denaturation temperature 69.4°C), finding 5–7 fold improved catalytic rates compared to the wild-type enzyme.
Increased interest in poly(ethylene terephthalate) (PET)-degrading enzymes (PETases) have generated efforts to find mutants with improved catalytic activity and thermostability. Here, we present a simple and fast method to determine relative enzyme kinetics through bulk absorbance measurements of released products over time. A thermostable variant of PETase from Ideonella sakaiensis was engineered (R280A S121E D186H N233C S282C) with a denaturation temperature of 69.4 ± 0.3 °C. This was used to assess the method's ability to determine relative enzyme kinetics across variants and reveal structure-function relationships. Measurements at 24 and 72 h at 400 nM of enzyme suggest that the mutations improved catalytic rates 5- to 7-fold. On the contrary, kinetic analyses of the thermostable variant and wild-type reveal different reaction trajectories despite similar maximum catalytic rates, resulting in higher product accumulation from the thermostable variant over time. The results of the assay support the necessity for kinetic measurements to determine relationships between sequence and function for IsPETase and other PET hydrolases.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.