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In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of Synechocystis sp. Using an Ultrasound-Enhanced ATR-FTIR Spectroscopy Probe
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Researchers developed an in-situ method to measure the production of polyhydroxybutyrate (PHB), a biodegradable plastic made by cyanobacteria, directly inside photobioreactors during cultivation. This advance could help scale up biological production of biodegradable plastics as a more sustainable alternative to petroleum-based plastics.
Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors.
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A Review of PHB Production by Cyanobacteria and Its Applications
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This review examines cyanobacteria as photoautotrophic producers of polyhydroxybutyrate (PHB) bioplastic, summarizing how nutrient-stress conditions stimulate PHB accumulation and evaluating the cost-effectiveness and sustainability of using cyanobacteria as an alternative to conventional feedstocks for biodegradable plastic production.
Absolute quantitation of poly(R)-3-hydroxybutyric acid using spectrofluorometry in recombinant Escherichia coli
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Researchers developed and validated a spectrofluorometry method for absolute quantification of polyhydroxybutyric acid (PHB) — a biodegradable bioplastic — in microbial cells. The method provides a faster and more accessible alternative to traditional gravimetric approaches for screening bacteria that produce biodegradable plastics.
Photosynthetic poly-β-hydroxybutyrate accumulation in unicellular cyanobacterium Synechocystis sp. PCC 6714
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This study investigated Synechocystis sp. PCC 6714 as a cyanobacterium for producing the biodegradable plastic precursor poly-beta-hydroxybutyrate (PHB) directly from CO2 via photosynthesis. Photosynthetic PHB production could reduce costs and improve the sustainability of this bioplastic alternative to petroleum-derived polymers.
Poly-β-hydroxybutyrate production by Synechocystis MT_a24 in a raceway pond using urban wastewater
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This study tested whether cyanobacteria could produce a biodegradable plastic called PHB while growing in urban wastewater outdoors. Researchers found that the organisms achieved one of the highest PHB yields reported for this type of production, reaching nearly 24% of their dry weight. The approach also removed significant amounts of nitrogen and phosphorus from the wastewater, suggesting a promising path toward sustainable bioplastic production.
Production of polyhydroxybutyrate (PHB) in Scenedesmus acutus using a low-cost substrate
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Researchers cultivated the microalga Scenedesmus acutus on low-cost substrates to produce polyhydroxybutyrate (PHB), a biodegradable bioplastic, demonstrating a cost-effective route toward replacing conventional petroleum-based plastics that persist as microplastic pollutants.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.