Using Nano Zero-Valent Iron Supported on Diatomite to Remove Acid Blue Dye: Synthesis, Characterization, and Toxicology Test

This work aimed to synthesize and characterize nanoscale zero-valent iron (nZVI), supported on diatomaceous earth (DE) at two different molar concentrations, 3 and 4 M (nZVI-DE-1 nZVI-DE-2), to test the decolorization treatment of acid blue dye (AB) and perform a toxicological test using zebrafish. The synthesis of the nanoparticles was obtained using the chemical reduction method. The material was fully characterized by X-ray diffraction, scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), and transmission electron microscopy and specific surface area (BET). The results showed spherical forms in clusters between 20 and 40 nm of zero-valent iron supported on diatomaceous earth. The removal of 1 g/L of AB from water treated with nZVI-DE-1 and nZVI-DE-2 reached the decolorization of 90% and 98% of all dye. By contrast, controls such as nZVI and DE-1 and DE-2 removed 40%, 37%, and 24% of the dye. Toxicological analysis using zebrafish showed that AB causes a severe defect in development, and embryos die after exposure. However, the water samples treated with nZVI-DE-1 and nZVI-DE-2 are not harmful to the zebrafish embryos during the first 24 h. However, all embryos exposed to the new material for more than 48 hpf had cardiac edema, smaller eyes, and curved and smaller bodies with less pigmentation.