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Detection of Microplastic Residues- Developing a Method for Phtalates in Honey
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This pilot study developed an analytical method for detecting phthalate plasticizers in honey, targeting five common phthalate compounds including diethyl phthalate and DEHP. The method was developed as part of research on detecting microplastic-related chemical residues in food products.
In this pilot study, a method for the determination of phthalates in honey was developed. The following phthalates are included: diethyl phthalate, di-isobutyl phthalate, di-n-butyl phthalate, bis (2-Ethylhexyl) phthalate, and di- (n-octyl) phthalate. For the preparation of the samples, the method of liquid-liquid extraction with hexane with an ultrasonic bath was used. The analysis of the prepared samples was performed using gas chromatography and a mass detector. The method is reliable, sensitive, and reproducible with a detection limit of 0.28 - 1.38 ug/kg. This paper presents the results of testing samples of honey stored in glass and plastic packaging for three years in order to determine the migration of phthalates. Dimethyl phthalate was not found in the tested samples. Diethyl phthalate in glass samples from glass packaging was below the LOQ, and it ranged from 2.1 to 4.11 µg / kg in the samples of honey in plastic packaging. The values of di-isobutyl phthalate, di-n-butyl phthalate and bis (2-ethylhexyl) phthalate ranged between 5.32 and 22.60 µg / kg respectively;1.32 – 27.80 µg/kg; 4.45-19.19 µg/kg; di-(n-octyl) phthalate and di-methyl phthalate were < LOQ
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