We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Identification of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters
AI summary Read the abstract
Researchers identified two enzymes produced by a cold-adapted fungus (Geomyces sp. B10I) that are responsible for breaking down biodegradable polyester plastics like PCL and PBS. These novel enzymes belong to a class called hydrolases and appear distinct from other known plastic-degrading fungal enzymes, making them potentially useful for developing new plastic biodegradation tools.
Cold-adapted filamentous fungal strain Geomyces sp. B10I has been reported to decompose polyesters such as poly(e-caprolactone) (PCL), poly(butylene succinate) (PBS) and poly(butylene succinate-co-butylene adipate) (PBSA). Here, we identified the enzymes of Geomyces sp. B10I, which appear to be responsible for its biodegradation activity. We compared their amino acid sequences with sequences of well-studied fungal enzymes. Partial purification of an extracellular mixture of the two enzymes, named hydrGB10I and chitGB10I, using ammonium sulfate precipitation and ionic exchange chromatography gave 14.16-fold purity. The amino acid sequence of the proteins obtained from the MALDI-TOF analysis determined the molecular mass of 77.2 kDa and 46.5 kDa, respectively. Conserved domain homology analysis revealed that both proteins belong to the class of hydrolases; hydrGB10I belongs to the glycosyl hydrolase 81 superfamily, while chitGB10I contains the domain of the glycosyl hydrolase 18 superfamily. Phylogenetic analysis suggests a distinct nature of the hydrGB10I and chitGB10I of Geomyces sp. B10I when compared with other fungal polyester-degrading enzymes described to date.
More Papers Like This
Hydrolytic Degradation of Polyethylene Terephthalate by Cutinase Enzyme Derived from Fungal Biomass–Molecular Characterization
AI summary Read the abstract
Researchers isolated cutinase and lipase enzymes from Aspergillus tamarii and Penicillium crustosum fungi and demonstrated their ability to catalyze hydrolytic degradation of PET plastic, offering a potential biological route for plastic waste breakdown.
Plastic biodegradation: Frontline microbes and their enzymes
AI summary Read the abstract
Researchers reviewed microbial biodegradation of synthetic plastics — including PE, PP, PS, and PET — cataloguing the insects, bacteria, and fungi capable of breaking down these polymers along with the enzymatic mechanisms involved, and outlining paths forward including metabolic pathway engineering and molecular cloning to improve degradation rates.
Fungal Enzymes Involved in Plastics Biodegradation
AI summary Read the abstract
Researchers reviewed the current literature on fungal enzymes capable of degrading various types of plastic polymers. The study cataloged different enzyme classes including laccases, peroxidases, and cutinases, describing their characteristics and efficacy against specific plastics. Evidence indicates that fungi offer a promising biological approach to plastic biodegradation due to their diverse array of enzymes specialized in breaking down recalcitrant substances.
Fungal potential for the degradation of petroleum-based polymers: An overview of macro- and microplastics biodegradation
AI summary Read the abstract
This review examines the potential of fungi to break down petroleum-based plastics, highlighting their unique ability to produce enzymes capable of degrading complex polymers. Researchers found that certain fungal species can use plastic materials as their sole carbon and energy source, offering a promising biological approach to plastic waste remediation. The study calls for further research on novel fungal isolates and molecular techniques to enhance plastic biodegradation processes.
Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium
AI summary Read the abstract
Researchers identified BgP, a cutinase-like polyesterase enzyme from a deep-sea sponge-derived actinobacterium, which can hydrolyze synthetic polyesters including PET plastic, highlighting marine bacteria as a promising source of plastic-degrading enzymes.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.