We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Sugar Beet Molasses as a Potential C-Substrate for PHA Production by Cupriavidus necator
Summary
Sugar beet molasses hydrolysates were evaluated as low-cost carbon substrates for polyhydroxyalkanoate (PHA) bioplastic production by the bacterium Cupriavidus necator, finding that the strain could efficiently convert molasses sugars into PHA at competitive yields. The study supports agricultural byproduct streams as economically viable feedstocks for scaling up microbially produced biodegradable plastics.
To increase the availability and expand the raw material base, the production of polyhydroxyalkanoates (PHA) by the wild strain Cupriavidus necator B-10646 on hydrolysates of sugar beet molasses was studied. The hydrolysis of molasses was carried out using β-fructofuranosidase, which provides a high conversion of sucrose (88.9%) to hexoses. We showed the necessity to adjust the chemical composition of molasses hydrolysate to balance with the physiological needs of C. necator B-10646 and reduce excess sugars and nitrogen and eliminate phosphorus deficiency. The modes of cultivation of bacteria on diluted hydrolyzed molasses with the controlled feeding of phosphorus and glucose were implemented. Depending on the ratio of sugars introduced into the bacterial culture due to the molasses hydrolysate and glucose additions, the bacterial biomass concentration was obtained from 20-25 to 80-85 g/L with a polymer content up to 80%. The hydrolysates of molasses containing trace amounts of propionate and valerate were used to synthesize a P(3HB-co-3HV) copolymer with minor inclusions of 3-hydroxyvlaerate monomers. The introduction of precursors into the medium ensured the synthesis of copolymers with reduced values of the degree of crystallinity, containing, in addition to 3HB, monomers 3HB, 4HB, or 3HHx in an amount of 12-16 mol.%.
Sign in to start a discussion.
More Papers Like This
Polyhydroxyalkanoate production by Cupriavidus necator with inedible rice
Researchers optimized polyhydroxyalkanoate (PHA) production by Cupriavidus necator using inedible rice hydrolysate as a carbon source, achieving 4.82 g/L PHA at 68.6% cell content after 72 hours at 5-L scale, comparable to glucose-fed conditions (4.74 g/L at 77.6%). The study demonstrates that inedible rice hydrolysate is a viable low-cost feedstock for biodegradable PHA polymer production as an alternative to petroleum-based plastics.
Sustainable bioconversion of excess grape must into polyhydroxyalkanoates by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034
Researchers tested surplus red and white grape musts as low-cost fermentation feedstocks for polyhydroxyalkanoate (PHA) bioplastic production using two bacterial strains, finding that Cupriavidus necator DSM 545 achieved up to 61.5% PHB content of cell dry weight in batch cultures and sustained production under fed-batch conditions, supporting grape must as a promising circular-economy substrate for bioplastics.
Properties of Degradable Polyhydroxyalkanoates (PHAs) Synthesized by a New Strain, Cupriavidus necator IBP/SFU-1, from Various Carbon Sources
Researchers characterized a new Cupriavidus necator strain capable of producing polyhydroxyalkanoates (PHAs) under both autotrophic and organotrophic conditions from diverse carbon sources including sugars, glycerol, fatty acids, and plant oils. The carbon source strongly influenced PHA composition and properties, with oleic acid and fructose yielding the highest polymer contents of over 78-80%.
Exploiting sugar-rich feedstocks for sustainable polyhydroxyalkanoate production
Researchers investigated the use of sugar-rich agricultural feedstocks for sustainable production of polyhydroxyalkanoates (PHAs), evaluating these bacterial biopolymers as biodegradable alternatives to petrochemical plastics that contribute to microplastic pollution.
A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources
This review summarizes strategies for enhancing poly(3-hydroxybutyrate) bioplastic production by Cupriavidus necator using low-cost carbon sources, covering metabolic engineering approaches and fermentation optimization as sustainable alternatives to conventional plastics.