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Production and optimization of bioplastic (Polyhydroxybutyrate) from Bacillus cereus strain SH-02 using response surface methodology

BMC Microbiology 2022 58 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Shereen M. Hamdy, Shereen M. Hamdy, Amal W. Danial, Sanaa M. F. Gad El-Rab, Ahmed A. M. Shoreit, Ahmed A. M. Shoreit, Abd El‐Latif Hesham

Summary

Researchers optimized conditions for a bacterium called Bacillus cereus to produce polyhydroxybutyrate (PHB) — a biodegradable plastic made naturally by bacteria — achieving high yields using a statistical optimization method called response surface methodology. PHB is a promising alternative to conventional petroleum-based plastics because it breaks down in the environment, potentially reducing long-term microplastic accumulation.

PHB-producing strain was identified as Bacillus cereus SH-02 (OM992297). Under optimum conditions from RSM analysis, the maximum PHB content and concentration of this strain can reach (3100.799 mg/L and 28.799%); respectively. FTIR, NMR, and Mass spectrometry were used to confirm the polymer as PHB. Our results demonstrated that optimization using RSM is one of the strategies used for reducing the production cost. RSM can determine the optimal factors to produce the polymer in a better way and in a larger quantity without consuming time.

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