We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
A new approach to extracting biofilm from environmental plastics using ultrasound-assisted syringe treatment for isotopic analyses
Summary
Researchers developed an ultrasound-assisted syringe extraction method for recovering biofilms from environmental plastic debris, enabling stable carbon and nitrogen isotope analysis and radiocesium quantification, and found that river-mouth plastisphere biofilms in Japan carried up to 820 Bq/kg of radiocesium, demonstrating that plastic-associated biofilms can serve as vectors for radionuclide transport in coastal environments.
Plastics are one of the ubiquitous and artificial types of substrates for microbial colonization and biofilm development in the aquatic environment. Characterizing plastic-associated biofilms is key to the better understanding of organic material and mineral cycling in the "Plastisphere"-the thin layer of microbial life on plastics. In this study, we propose a new method to extract biofilms from environmental plastics, in order to evaluate the properties of biofilm-derived organic matter through stable carbon (δC) and nitrogen (δN) isotope signatures and their interactions with radionuclides especially radiocesium (Cs). The extraction method is simple and cost-effective, requiring only an ultrasonic bath, disposable plastic syringes, and a freeze drier. After ultrasound-assisted separation from the plastics, biofilm samples were successfully collected via a sequence of syringe treatments, with less contamination from plastics and other mineral particles. Effective removal of small microplastics from the experimental suspension was satisfactorily achieved using the method with syringe treatments. Biofilm-derived organic matter samples (14.5-65.4 mg) from four river mouths in Japan showed Cs activity concentrations of <75 to 820 Bq·kg biofilm (dw), providing evidence that environmental plastics, mediated by developed biofilms, serve as a carrier for Cs in the coastal riverine environment. Significant differences in the δC and δN signatures were also obtained for the biofilms, indicating the different sources, pathways, and development processes of biofilms on plastics. We demonstrate here a straightforward method for extracting biofilms from environmental plastics; the results obtained with this method could provide useful insights into the plastic-associated nutrient cycling in the environment.
Sign in to start a discussion.
More Papers Like This
Biofilm-enhanced adsorption of strong and weak cations onto different microplastic sample types: Use of spectroscopy, microscopy and radiotracer methods
Researchers used radiotracer, spectroscopy, and microscopy methods to show that biofilm-coated environmental plastics adsorb radioactive cesium and strontium — radionuclides associated with nuclear releases — though at rates much lower than natural sediments, confirming that plastics act as a minor but measurable sink for environmental radioactivity.
Initial data on adsorption of Cs and Sr to the surfaces of microplastics with biofilm
Researchers measured adsorption of radiocesium and radiostrontium onto weathered microplastics deployed in freshwater, estuarine, and marine environments, finding that distribution coefficients were approximately two to three orders of magnitude lower than for sediment reference values. Despite the lower adsorption, the buoyancy and mobility of plastics suggest they may still function as a significant radionuclide reservoir in aquatic systems.
Unfolding the interaction of radioactive Cs and Sr with polyethylene-derived microplastics in marine environment
Researchers investigated how polyethylene microplastics in the marine environment interact with radioactive cesium and strontium. They found that as microplastics age in seawater and develop biofilms, their ability to absorb these radioactive elements increases significantly. The study provides evidence that microplastics could act as previously unrecognized carriers of radioactive contamination in ocean environments.
Unfolding the interaction of radioactive Cs and Sr with polyethylene-derived microplastics in marine environment
A mesocosm study examined how radioactive cesium and strontium interact with pristine, radiation-exposed, and marine-weathered polyethylene microplastics, finding that environmental aging—through biofilm formation and surface roughening—significantly increased the plastic particles' capacity to sorb radioactive contaminants.
Marine microplastics fuel long-range transport of radioactive nuclides: A review
This review examines how marine microplastics adsorb radioactive nuclides and transport them over long distances, discussing the implications of plastic-facilitated radionuclide dispersal for ocean monitoring and the compounding environmental risks from co-occurring plastic and nuclear contamination.