Rhamnolipid-Enhanced ZVI-Activated Sodium Persulfate Remediation of Pyrene-Contaminated Soil

In soil, polycyclic aromatic hydrocarbons (PAHs) are tightly bound to organic components, but surfactants can effectively transform them from a solid to a liquid phase. In this study, the biosurfactant rhamnolipid (RL) was selected as the eluent; shaking elution in a thermostatic oscillator improved the elution rate of pyrene, and the effects of RL concentration, temperature, and elution time on the elution effect were compared. After four repeated washings, the maximum elution rate was 75.6% at a rhamnolipid concentration of 20 g/L and a temperature of 45 °C. We found that 38 μm Zero-Valent Iron (ZVI) had a higher primary reaction rate (0.042 h^-1), with a degradation rate of 94.5% when 3 g/L ZVI was added to 21 mM Na₂S₂O₈ at 60 °C. Finally, electron paramagnetic resonance (EPR) detected DMPO-OH and DMPO-SO₄ signals, which played a major role in the degradation of pyrene. Overall, these results show that the combination of rhamnolipid elution and persulfate oxidation system effectively remediated pyrene-contaminated soil and provides some implications for the combined remediation with biosurfactants and chemical oxidation.