Mycobacteriaceae Mineralizes Micropolyethylene in Riverine Ecosystems

Microplastic (MP) contamination is a serious global environmental problem. Plastic contamination has attracted extensive attention during the past decades. While physiochemical weathering may influence the properties of MPs, biodegradation by microorganisms could ultimately mineralize plastics into CO₂. Compared to the well-studied marine ecosystems, the MP biodegradation process in riverine ecosystems, however, is less understood. The current study focuses on the MP biodegradation in one of the world's most plastic contaminated rivers, Pearl River, using micropolyethylene (mPE) as a model substrate. Mineralization of ¹³C-labeled mPE into ¹³CO₂ provided direct evidence of mPE biodegradation by indigenous microorganisms. Several <i>Actinobacteriota</i> genera were identified as putative mPE degraders. Furthermore, two <i>Mycobacteriaceae</i> isolates related to the putative mPE degraders, <i>Mycobacterium</i> sp. mPE3 and <i>Nocardia</i> sp. mPE12, were retrieved, and their ability to mineralize ¹³C-mPE into ¹³CO₂ was confirmed. Pangenomic analysis reveals that the genes related to the proposed mPE biodegradation pathway are shared by members of <i>Mycobacteriaceae</i>. While both <i>Mycobacterium</i> and <i>Nocardia</i> are known for their pathogenicity, these populations on the plastisphere in this study were likely nonpathogenic as they lacked virulence factors. The current study provided direct evidence for MP mineralization by indigenous biodegraders and predicted their biodegradation pathway, which may be harnessed to improve bioremediation of MPs in urban rivers.