Beyond the exposure phase: Microplastic depuration and experimental implications

Currently, most studies focus on the effect of microplastics (MPs) in the exposure phase, but pay limited attention to the depuration phase. Depuration is a promising practice to achieve safe aquaculture production, which is also helpful to understand the long-term impact of MPs. Therefore, investigating the post-exposure scenarios of MPs has great practical significance. In order to provide implications for future research, this work attempted to systematize the current findings and knowledge gaps regarding the depuration of MPs. More specifically, three methods, including direct fitting, one-compartment kinetic model and interval observation, for estimating the retention time of MPs to further determine the minimum depuration time were introduced, in which the one-compartment kinetic model could also be used to calculate the depuration rate constant and biological half-life of MPs. Moreover, the post-exposure effect of MPs generally presented three scenarios: incomplete reversal (legacy effect), return to control level (recovery) and stimulatory response (hormesis-like effect). In addition, the possible tissue translocation of MPs, the influence of food abundance and body shape on MPs egestion, and the potential interaction with environmental factors, have aroused great scientific concerns and need further exploration and clarification.