Microplastics existence intensified bloom of antibiotic resistance in livestock feces transformed by black soldier fly

Efficient degradation of residual antibiotics in livestock and poultry feces by black soldier flies (BSFs) has been widely reported. Nevertheless, the effects of widely detected microplastics in feces on the dynamic reduction of antibiotics and the transfer of gut bacterial resistome remain unclear. In this study, red fluorescence-labeled microplastics are observed to be abundantly distributed in BSFs gut, which caused epithelial cell damage along with gut peristalsis and friction, thereby releasing reactive oxygen species and activating the antioxidant enzyme system. In addition, they result in not only in inflammatory cytokine release to induce gut inflammation, but fecal hardening because of mucus released from the BSFs, thereby hindering organic mineralization and antibiotic degradation. Besides, the gut pathogenic bacteria easily obtain growth energy and crowded out ecological niches by reducing nitrate produced by inflammatory host cells to nitrite with nitrate reductase. Consequently, linear discriminant analysis effect size and detrended correspondence analysis found that microplastic intake significantly reshape the microbial community structure and cause the significant reduction of several important organic-decomposing bacteria and probiotics (e.g., Pseudomonadales, Coriobacteriales, Lachnospirales, and Ruminococcaceae). In addition, a large number of pathogenic bacteria (e.g., Enterococcaceae, Hungateiclostridiaceae, and Clostridia) are enriched in feces and BSFs gut. Weighted correlation network analysis and bubble diagram analysis indicate that microplastic intake intensified gut colonization of pathogenic bacteria carrying antibiotic-resistant genes/mobile genetic elements, driving the bloom of antibiotic resistance in transformed fecal piles. Therefore, microplastics in feces should be isolated as much as possible before insect transformation.