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Graphene oxide disruption of homeostasis and regeneration processes in freshwater planarian Dugesia japonica via intracellular redox deviation and apoptosis

Ecotoxicology and Environmental Safety 2022 14 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Willie J.G.M. Peijnenburg, Changjian Xie, Willie J.G.M. Peijnenburg, Xiaowei Li, Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Changjian Xie, Iseult Lynch Peng Zhang, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Xiaowei Li, Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Zhiling Guo, Iseult Lynch Zhiling Guo, Zhiling Guo, Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Shujing Zhang, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Peng Zhang, Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Yuling Dong, Yuling Dong, Yuling Dong, Yuling Dong, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Iseult Lynch Zhiling Guo, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Shujing Zhang, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Qiuxiang Pang, Iseult Lynch Ao Li, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Peng Zhang, Peng Zhang, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Shujing Zhang, Willie J.G.M. Peijnenburg, Qiuxiang Pang, Iseult Lynch Zhiling Guo, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Iseult Lynch Shan Ma, Shan Ma, Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Changjian Xie, Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Peng Zhang, Zhiling Guo, Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Qiuxiang Pang, Jianing Xu, Willie J.G.M. Peijnenburg, Iseult Lynch Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Qiuxiang Pang, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Peng Zhang, Iseult Lynch Willie J.G.M. Peijnenburg, Zhiling Guo, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Peng Zhang, Iseult Lynch Peng Zhang, Willie J.G.M. Peijnenburg, Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Peng Zhang, Peng Zhang, Peng Zhang, Peng Zhang, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Willie J.G.M. Peijnenburg, Zhiling Guo, Zhiling Guo, Iseult Lynch Iseult Lynch Iseult Lynch Willie J.G.M. Peijnenburg, Zhiling Guo, Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch Iseult Lynch

Summary

Graphene oxide was found to disrupt homeostasis and regeneration processes in the freshwater planarian Dugesia japonica, with exposed animals showing impaired wound healing and tissue regeneration at low concentrations. The study establishes planarians as a sensitive model for assessing nanomaterial toxicity with relevance to regenerative biology.

Body Systems

Immune Nervous

Study Type Environmental

The aquatic system is a major sink for engineered nanomaterials released into the environment. Here, we assessed the toxicity of graphene oxide (GO) using the freshwater planarian Dugesia japonica, an invertebrate model that has been widely used for studying the effects of toxins on tissue regeneration and neuronal development. GO not only impaired the growth of normal (homeostatic) worms, but also inhibited the regeneration processes of regenerating (amputated) worms, with LC<sub>10</sub> values of 9.86 mg/L and 9.32 mg/L for the 48-h acute toxicity test, respectively. High concentration (200 mg/L) of GO killed all the worms after 3 (regenerating) or 4 (homeostasis) days of exposure. Whole-mount in situ hybridization (WISH) and immunofluorescence analyses suggest GO impaired stem cell proliferation and differentiation, and subsequently caused cell apoptosis and oxidative DNA damage during planarian regeneration. Mechanistic analysis suggests that GO disturbed the antioxidative system (enzymatic and non-enzymatic) and energy metabolism in the planarian at both molecular and genetic levels, thus causing reactive oxygen species (ROS) over accumulation and oxidative damage, including oxidative DNA damage, loss of mitochondrial membrane integrity, lack of energy supply for cell differentiation and proliferation leading to retardance of neuron regeneration. The intrinsic oxidative potential of GO contributes to the GO-induced toxicity in planarians. These data suggest that GO in aquatic systems can cause oxidative stress and neurotoxicity in planarians. Overall, regenerated tissues are more sensitive to GO toxicity than homeostatic ones, suggesting that careful handling and appropriate decisions are needed in the application of GO to achieve healing and tissue regeneration.

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